HPLC Columns

Columns used for separation of biomolecules and organic or inorganic analytes; includes normal phase, reversed phase, UHPLC, LC-MS, HILIC, mixed-mode, ion exchange, ligand exchange, and affinity column formats.

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166 – 180 of 28,775 results

166

MilliporeSigma™ Supelco™ SUPELCOSIL™ Suplex™ pKb-100 HPLC Column, 5 μm Particle Size

Suplex™ pKb-100 columns feature the same bonded phase functionality as SUPELCOSIL™ LC-ABZ columns.

Pricing & Availability
Specifications

Pore Size	120 Å
Particle Size	5 μm
Stationary Phase	Amide, Alkyl
Phase	Reversed Phase
Matrix	Amide, Alkyl Phase/Silica Gel/Spherical Particle Platform
Endcapped	No
Surface Area	170 m2/g
Product Line	Suplex

167

TSKgel™ ODS-120A HPLC Column, 5 μm Particle Size, Sold by MilliporeSigma™ Supelco™

TSKgel™ ODS-120A columns use a 150 Å pore size silica base support. The silica′s exclusion limit of 10,000 Da makes them a good choice for the reversed phase chromatography of peptides, small proteins, and environmental samples such as poly-aromatic hydrocarbons.

Pricing & Availability
Specifications

Pore Size	150 Å
Solvents	0 to 100% organic solvent
Stationary Phase	C18 (octadecyl)
Phase	Reversed Phase
Endcapped	No
Surface Area	200 m2/g
Particle Size	5 μm
Flow Rate	1.2 mL/min.
Carbon Load	20%
Matrix	Silica Particle Platform
Diameter (Metric)	4.6 mm
pH	2.0 - 7.5
Product Line	TSKgel
USP Type	L1

168

Thermo Scientific™ μPAC™ HPLC Columns

Learn more about your proteome, metabolome, and lipidome samples with Thermo Scientific™ μPAC™ HPLC columns.

Pricing & Availability
Specifications

Pore Size	100 to 200 Å
Stationary Phase	C18
Temperature	60°C
Phase	Reversed Phase
Endcapped	Yes
Packing Material	Silicon Chip
Column Type	Micro Pillar Array
pH	1.5 to 7
Interpillar Distance	2.5 μm
Porosity	59%
Pillar Diameter	5 μm
Max. Pressure	350 bar (5076 psi)

169

TSKgel™ CM-3SW HPLC Column, 10 μm Particle Size, Sold by MilliporeSigma™ Supelco™

TSKgel™ CM-3SW columns are packed with 5 micron, high efficiency, spherical silica beads which are surface modified with a weak cation exchanger. They are used for the separation and analysis of proteins, peptides, and other biologically active molecules. They have a larger pore size than the TSKgel™ CM-2SW columns.

Pricing & Availability

170

TSKgel™ SAX HPLC Column, 5 μm Particle Size, Sold by MilliporeSigma™ Supelco™

TSKgel™ SAX columns are packed with 5 μm porous spherical PS/DVB particles which are surface modified with a strong anion exchanger. They are used for the separation of isomerized sugars, alcohols, and low molecular weight organic acids.

Pricing & Availability

171

Thermo Scientific™ ProPac™ PA1 HPLC Columns

Efficiently analyze and purify hydrophilic anionic proteins and peptides with ProPac™ PA1 LC Columns.

Pricing & Availability
Specifications

Pore Size	Nonporous
Particle Size	10 μm
Flow Rate	0.9 to 1.5 mL/min
Stationary Phase	Alkyl Quaternary Amine
Phase	Ion Exchange
For Use With	BioLC Chromatography System
Packing Material	Spherical, Nonporous Polymer
Column Type	Ion Exchange
Quantity	Each
Green Features	FSC
Material	PEEK
Max. Pressure	5000 psi (345 bar)
Product Line	ProPac

172

Thermo Scientific™ BioBasic™ 8 HPLC Columns

Use this column for reversed-phase separation of proteins and other biomolecules.

Pricing & Availability
Specifications

Pore Size	300 Å
Particle Size	5 μm
Carbon Load	5%
Temperature	60°C
Phase	Reversed Phase
Endcapped	Yes
Column Type	Reversed Phase
pH	2 to 9
Surface Area	100 m²/g
Max. Pressure	5800 psi (400 bar)
Product Line	BioBasic
USP Type	L7

173

TSKgel™ G6000PW HPLC Column, 17 μm Particle Size, Sold by MilliporeSigma™ Supelco™

TSKgel™ G6000PW SEC columns can be used for the analysis of water-soluble polymers up to 8,000,000 Da molecular weight.

Pricing & Availability
Specifications

Pore Size	>1000 Å
Particle Size	17 μm
Solvents	≤20% organic solvent
Flow Rate	1.2 mL/min.
Stationary Phase	Hydroxylated Methacrylate
Temperature	80°C
Phase	Size Exclusion
Matrix	Polymer Particle Platform
Diameter (Metric)	7.5 mm
pH	2.0 - 12
Product Line	TSKgel
USP Type	L38, L39

174

TSKgel™ G2500PW HPLC Column, 12 μm Particle Size, Sold by MilliporeSigma™ Supelco™

TSKgel™ G2500PW SEC columns are for the analysis of water-soluble polymers with molecular weights of less than 3,000 Da.

Pricing & Availability
Specifications

Pore Size	<200 Å
Particle Size	12 μm
Solvents	≤20% organic solvent
Flow Rate	1.2 mL/min.
Stationary Phase	Hydroxylated Methacrylate
Temperature	80°C
Phase	Size Exclusion
Matrix	Polymer Particle Platform
Diameter (Metric)	7.5 mm
pH	2.0 - 12
Product Line	TSKgel
USP Type	L25, L38, L39

175

TSKgel™ DEAE-5PW HPLC Column, 10 μm Particle Size, Sold by MilliporeSigma™ Supelco™

TSKgel™ DEAE-5PW columns are packed with porous hydrophilic polymer beads which are surface modified with a weak anion exchanger. They are used for the separation and analysis of proteins, nucleotides, nucleosides and other biologically active molecules.

Pricing & Availability

176

MilliporeSigma™ Supelco™ Ascentis™ RP-Amide HPLC Column, 5 μm Particle Size

Ascentis™ RP-Amide is a new generation ultra low bleed, embedded polar group (EPG) phase that provides orthogonal selectivity and increased resolution for HPLC and LC-MS analysis of polar compounds. The Ascentis™ RP-Amide is the first choice in embedded polar group HPLC phases.

Pricing & Availability
Specifications

Pore Size	100 Å
Particle Size	5 μm
Stationary Phase	Amide, Alkyl
Phase	Reversed Phase
Matrix	Amide, Alkyl Phase/Fused-Core Particle Platform
Endcapped	Yes
pH	2 to 8
Technique	Fused-Core HPLC
Surface Area	450 m2/g
Product Line	Ascentis

177

Thermo Scientific™ POROS™ CaptureSelect™ HSA Affinty HPLC Columns

Analytical groups supporting biologic drug development require rapid, sensitive and precise methods for protein quantitation from cell culture, fermentation harvests, or purification intermediates.

Pricing & Availability
Specifications

Shipping Condition	Wet Ice
Pore Size	2000 Å
Stationary Phase	Human Serum Albumin affinity ligand, humanspecific
Column Type	Affinity
Quantity	1 Column
Material	PEEK
High-throughput Compatibility	Not High-throughput Compatible (Manual)
Max. Pressure	180 bar
Particle Size	20 μm
Flow Rate	Up to 5000 cm/hr.
Matrix	Polystyrenedivinylbenzene
Reactivity	Human
Target Molecule	HSA, HSA Fusion Proteins
Product Type	Column
Binding Capacity	2.6 mg/mL
Sample Type	Cell Culture Supernatants
Purification Target	HSA, HSA Fusion Proteins
Product Line	CaptureSelect, POROS

178

MilliporeSigma™ Supelco™ Ascentis™ Express ES-Cyano UHPLC Column, 2 μm Particle Size

The sterically protected, extensively endcapped diisopropyl-cyanopropylsilane stationary phase of Ascentis™ Express 2 μm ES-Cyano provides a stable, reversed-phase packing that can be used for basic, acidic, or neutral compounds.

Pricing & Availability
Specifications

Pore Size	90 Å
Particle Size	2 μm
Stationary Phase	Diisopropyl-Cyanopropylsilane
Phase	Reversed Phase
Matrix	Diisopropyl-Cyanopropylsilane Phase/Fused-Core Particle Platform
Endcapped	Yes
pH	1 to 8
Technique	Fused-Core UHPLC
Product Line	Ascentis

179

Thermo Scientific™ Hypersil™ MOS-2 C8 HPLC Columns

Use as a general-purpose C8 phase for acidic and neutral analytes, with decrease silanol activity

Pricing & Availability
Specifications

Pore Size	120 Å
Carbon Load	6.5%
Stationary Phase	C8
Temperature	60°C
Phase	Reversed Phase
Column Type	Reversed Phase
pH	2 to 8
Surface Area	170 m²/g
Max. Pressure	5800 psi (400 bar)
Product Line	Hypersil Classical
USP Type	L7

180

TSKgel™ NH2-100 DC Direct Connect HPLC Column, 3 μm Particle Size, Sold by MilliporeSigma™ Supelco™

TSKgel™ NH2-100 DC column connects directly to TSKgel™ reversed phase columns. The DC in TSKgel™ NH2-100 DC emphasizes this Direct Connect aspect.

Pricing & Availability

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